TEV Protease Protein

Catalog No. ABIN2018393

This Recombinant protein is produced in Escherichia coli (E. coli).

Quick Overview for TEV Protease Protein (ABIN2018393)

Target: TEV Protease

Protein Type: Recombinant

Origin: Tobacco Etch Virus (TEV)

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE analyses.

Product Details

Characteristics: 6 IU/μl
Unit Definition: One unit of TEV protease cleaves > 85 % of 3 μg of control substrate in 1 hour at pH 8.0 at 30 °C.

Sterility: Sterile

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Sterile liquid solution contains 50 mM Tris, 5 mM DTT, 50 % glycerol, pH 7.5.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Recombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20 °C from date of receipt.

Expiry Date: 12 months

Target Details

Target: TEV Protease

Target Type: Viral Protein

Background: Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30 °C, also it can be used at temperature as low as 4 °C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.Recombinant Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by proprietary chromatographic techniques.
Synonyms: rTEV, TEV, P1 protease

Molecular Weight: 28.4 kDa, observed by reducing SDS-PAGE.